干旱胁迫下多效唑浸种对多年生黑麦草种子萌发的影响

Effects of soaking seeds with paclobutrazol on seed germination of Lolium perenne under the simulated drought stress

以多年生黑麦草(Lolium perenne)的7个品种为供试材料,用不同质量浓度(0、50、100、200、300和400mg/L)的多效唑溶液浸泡处理种子,采用纸上发芽法(TP)测定在PEG-6000模拟干旱胁迫条件下多效唑浸种对7个黑麦草品种萌发和生长的影响,旨在寻找出提高黑麦草萌发期抗旱性的最佳浸种浓度,同时为提高草坪质量提供理论依据和参考。结果表明,在干旱胁迫条件下,50～400mg/L多效唑处理对黑麦草种子萌发、苗质量、根长和根质量表现为促进作用,且促进作用随着多效唑质量浓度升高先加强后减弱,但对胚芽的生长有显著的抑制作用(P<0.05),且抑制作用随着多效唑质量浓度升高而加强。综上所述,以100mg/L的多效唑增强黑麦草种子萌发阶段抗旱性的效果最佳。

Under the simulated drought stress condition with PEG,the TP method was used to determine the effect of different concentrations of paclobutrazol solution（0,50,100,200,300 and 400 mg/L） on seed germination of seven accessions of Lolium perenne to find out the optimal soaking concentration.This study showed that the paclobutrazol solution soaking seeds of L.perenne had a positive effect on seed germination,seedling quality,root length and root quality and the effectiveness of paclobutrazol firstly increased and then decreased as paclobutrazol concentration increased.However,he paclobutrazol solution soaking seeds significantly inhibited the germ growth of L.perenne（P0.05）,and this inhibition increased with the increase of paclobutrazol concentration under drought stress condition.In summary,100 mg/L paclobutrazol solution soaking seeds was the best for L.perenne to improve drought resistance at the germination phase.