外源NO对NaCl胁迫下燕麦幼苗抗氧化酶活性和生长的影响

Effects of exogenous nitric oxide on activity of antioxidative enzyme and growth of oat seedlings under salt stress

为了探讨外源NO是否能减轻盐胁迫对燕麦(Avena sativa)幼苗的伤害,以水培生长到四叶一心的燕麦幼苗为研究材料,在150mmol/L NaCl胁迫下,探讨了0.06mmol/L外源NO供体硝普钠(SNP)处理对白燕6号和内散2号2个燕麦品种幼苗叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性以及丙二醛(MDA)含量、质膜透性、叶绿素含量和植株干质量的影响。结果表明,NaCl胁迫下,施入外源NO可提高燕麦叶片SOD、CAT、POD和APX活性,降低MDA含量和质膜透性,并能缓解叶绿素含量的下降,提高植株干质量,从而减轻NaCl胁迫对燕麦幼苗的伤害;但外源NO对不同抗氧化酶活性影响不同,其中对提高SOD、CAT、APX活性的作用相对较大,而对提高POD活性的作用相对较小。

Under 150 mmol/L NaCl stress,a hydroponic experiment was conducted to investigate the effect of 0.06 mmol/L sodium nitroprusside（SNP） on antioxidative enzyme and growth of two kind of oat seedlings（Baiyan 6 and Neisan 2） at fourth leaves stage by measuring the MDA,SOD,CAT,POD,APX contents,electronic conductivity,chlorophyll contents in leaves and dry weight.The results of this study indicated that 0.06 mmol/L SNP increased the activities of SOD,CAT,POD,APX and the dry weight,and decreased MDA,chlorophyll content and membrane permeability.The antioxidant enzyme activities showed a different response to exogenous NO,in which exogenous NO had more effectiveness on SOD,CAT and APX than that on POD.