栗生灰黑孔菌合成漆酶最佳发酵条件的优化

Optimization of fermentation conditions of laccase of Melanoporia castanea

研究了碳源、氮源、诱导剂、金属离子、pH值、温度、转速、装液量、接种量等培养条件对栗生灰黑孔菌产漆酶的影响。利用Minitab15软件、Plackett-Burman PB实验设计和响应面分析法对栗生灰黑孔菌产漆酶的发酵条件进行优化。结果表明:最佳液态发酵培养基组分为玉米秆粉9.976 g/L,黄豆粉9 g/L,ZnSO4.7H2O 0.3μmol/L,对苯二胺0.5 mmol/L;最佳培养条件为:温度28℃,转速180 r/min,pH值5.913,装液量60 mL/250 mL,接种量10%。在此条件下培养漆酶酶活可达431 U/mL。

The experiment studies the influence of culture conditions（carbon source,nitrogen source,revulsant,metallic ion,pH,temperature,rotation speed,the volume of liquid,inoculation amount of fungi） on the laccase production by Melanoporia castanea.Minitab15 software,and Plackett-Burman PB experimental design and response surface analysis were used to optimize the fermentation conditions of laccase of Melanoporia castanea.The results indicated that optimal liquid composition of fermentation medium：corn stalk powder 9.976 g/L,soybean meal 9 g/L,ZnSO4·7H2O 0.3 μmol/L,p-phenylenediamine 0.5 mmol/L.Optimal culture conditions are： temperature 28 ℃,rotation speed 180 r/min,pH 5.913,liquid volume 60mL/250mL,and inoculum 10%.Under the above culture conditions,laccase activity can be up to 431 U/mL.