甾体激素对神经母细胞株SK-N-SH细胞摄取甘氨酸的快速作用

THE RAPID EFFECTS OF STEROIDS ON GLYCINE UPTAKE IN NEUROBLASTOMA CELL STRAIN SK N SH CELLS 

本研究观察了SKNSH细胞摄取甘氨酸的一般情况,并进一步研究了甾体激素对SKNSH细胞摄取甘氨酸的快速作用。结果显示:SKNSH细胞高亲和力的甘氨酸摄取是Na+和Cl-依赖性的。皮质酮、孕酮和地塞米松对这种摄取有快速促进作用,雌二醇和脱氧皮质酮无明显作用,表明甾体激素快速作用有特异性。皮质酮作用在10-9～10-6mol/L范围内呈浓度依赖性。皮质酮快速作用不受蛋白质合成抑制剂影响。耦联牛血清白蛋白后,快速作用仍然存在。但细胞外液无Ca2+影响甾体激素快速作用。结果提示皮质酮。

In the present study, glycine uptake in SK N SH cells was determined with liquid scintillation technique, and the rapid effects of steroids on glycine uptake in SK N SH cells were investigated. The results were as follows. High affinity glycine uptake in SK N SH cells was dependent on Na + and Cl -. Corticosterone (CORT), progesterone (P) and dexamethasone (DEX) had rapid effects on the glycine uptake. Since estradiol (E2) and deoxycorticosterone (DOC) had no effects, it was suggested that the rapid effects of steroids were specific. The rapid effects of CORT were concentration dependent in a range of 10 -9 ～10 -6 mol/L. The rapid effects were not affected by the inhibitor of protein synthesis and persisted even when CORT was conjugated with bovine serum album, but attenuated when Ca 2+ was absent in the external medium. The results suggest that the steroid effect on glycine uptake in SK N SH cells was nongenomicly mediated.