摘要
本文用细胞贴附式和内面向外式的膜片箝单通道记录方式研究了小鼠骨髓基质细胞K+通道的动力学特性以及重组人白细胞介素1β(IL1β)对通道的影响,发现了骨髓基质细胞膜上存在一类性质类似于延迟整流的K+通道的电压依赖性K+通道,单通道电导为167±14pS,通道的动力学特性具显著的电压依赖性。1000U/mlIL1β使单通道电导增加到261±36pS,显著增加通道的开放概率,延长开放时间τo2,缩短关闭时间τc2,并诱导通道出现多级开放。研究表明K+通道的激活参与了细胞因子IL1的生物信号转导。
In the present study, both cell attached and inside out mode of patch clamp technique were applied to detect the effect of recombinant human interleukin 1β (IL 1β) on K + channels of mice bone marrow stromal cells. A 16 7 pS voltage dependent K + channel resembling the delayed rectifier K + channel in excitable cells was identified and characterized. Under stimulation of IL 1β (1?000 U/ml), the single channel conductance increased to 26 1±3 6 pS ( P <0 01). IL 1β also markedly increased the open time constant and the open probability, but decreased the closed time constant as compared to the same patches without IL 1β. In addition, IL 1β induced multi channel activities in about 80 percent patches by inducing more K + channels of the same type open. These results suggest that activation of K + channels plays a role in signal transduction of interleukin 1.
出处
《生理学报》
CAS
CSCD
北大核心
1999年第6期637-644,共8页
Acta Physiologica Sinica
基金
广东省自然科学基金!资助项目 (No 96 0 36 6 )
关键词
白细胞介素-1
钾通道
膜片箝
信号转导
骨髓细胞
interleukin 1
bone marrow stromal cell
K + channels
patch clamp
signal transduction
