摘要
目的:探讨健脾化瘀方含药血清对人结肠癌细胞LoVo凋亡的影响。方法:①应用MTT法检测健脾化瘀方含药血清对人结肠癌细胞LoVo的增殖抑制作用;②运用TUNEL法和AnnexinV/PI法检测LoVo细胞的凋亡率。结果:①健脾化瘀方含药血清高浓度(20%)可抑制LoVo细胞的增殖(P<0.01);②健脾化瘀方含药血清作用细胞48h后可以诱导细胞凋亡。结论:健脾化瘀方含药血清能抑制结肠癌细胞LoVo的增殖能力,并诱导细胞凋亡。
Objective:This study was designed to explore the effect of medicated serum of Jianpi Huayu prescription in vitro on the apoptosis of human colon cell line LoVo.Methods:①After human colon cell line LoVo was treated with medicated serum of Jianpi Huayu prescription at different concentrations,cell proliferation capability was assessed by MTT assay.②TUNEL and AnnexinV/PI double staining method were used to observe the early apoptosis induced by different concentrations of medicated serum of Jianpi Huayu Fang in human colon cell line LoVo.Results:①The high concentration(20%) of medicated serum of Jianpi Huayu prescription significantly inhibited the proliferation of LoVo cells(P0.01).②The medicated serum of Jianpi Huayu prescription applying on human colon cell line LoVo for 48h,can induce the apoptosis of human colon cell line LoVo with a degree of concentration-dependence.Conclusion:①Jianpi Huayu prescription can inhibit the proliferation of human colon cell line LoVo.③Jianpi Huayu prescription can induce the apoptosis of human colon cell line LoVo.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第12期2355-2356,I0017,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
江苏高校优势学科建设工程资助项目
江苏省中医药领军人才项目(LJ200908)
关键词
结肠癌
细胞增殖
细胞凋亡
健脾化瘀方
含药血清
colon carcinoma
cell proliferation
cell apoptosis
Jianpi Huayu prescription
medicated serum