健脾化瘀方含药血清对人结肠癌细胞LoVo凋亡的影响

Effects Serum with Jianpi Huayu Prescription on Apoptosis of Human Colon Cancer Cell Lovo

目的:探讨健脾化瘀方含药血清对人结肠癌细胞LoVo凋亡的影响。方法:①应用MTT法检测健脾化瘀方含药血清对人结肠癌细胞LoVo的增殖抑制作用;②运用TUNEL法和AnnexinV/PI法检测LoVo细胞的凋亡率。结果:①健脾化瘀方含药血清高浓度(20%)可抑制LoVo细胞的增殖(P<0.01);②健脾化瘀方含药血清作用细胞48h后可以诱导细胞凋亡。结论:健脾化瘀方含药血清能抑制结肠癌细胞LoVo的增殖能力,并诱导细胞凋亡。

Objective：This study was designed to explore the effect of medicated serum of Jianpi Huayu prescription in vitro on the apoptosis of human colon cell line LoVo.Methods：①After human colon cell line LoVo was treated with medicated serum of Jianpi Huayu prescription at different concentrations,cell proliferation capability was assessed by MTT assay.②TUNEL and AnnexinV/PI double staining method were used to observe the early apoptosis induced by different concentrations of medicated serum of Jianpi Huayu Fang in human colon cell line LoVo.Results：①The high concentration（20%） of medicated serum of Jianpi Huayu prescription significantly inhibited the proliferation of LoVo cells（P0.01）.②The medicated serum of Jianpi Huayu prescription applying on human colon cell line LoVo for 48h,can induce the apoptosis of human colon cell line LoVo with a degree of concentration-dependence.Conclusion：①Jianpi Huayu prescription can inhibit the proliferation of human colon cell line LoVo.③Jianpi Huayu prescription can induce the apoptosis of human colon cell line LoVo.