原位杂交检测大鼠前庭代偿中GAP-43mRNA水平

Checking GAP-43mRNA Levels of the Vestibular Nucleus in the Rat Vestibular Compensation by in situ Hybridization Method.

用ＤＩＧ 标记的ＧＡＰ４３ ｃＤＮＡ 为探针， 以大鼠海马切片作阳性对照， 使用原位杂交方法检测了大鼠迷路损毁５ 、１２ 、２０ 和３０ ｄ 后前庭核区ＧＡＰ４３ ｍ ＲＮＡ 水平的变化． 结果表明， 迷路损毁后前庭核区ｍ ＲＮＡ 水平升高．原位杂交的应用， 为前庭代偿中轴突发芽， 突触重组的神经可塑性研究打下了方法学基础．

Using DIG (digoxigenin) labeled GAP 43 cDNA as probe, the in situ hybridization method (ISHM) was set up with rat hippocampus slides as positive controls. The change of GAP 43 mRNA levels in the vestibular nucleus area were investigated by ISHM in the labyrinthectomy rats at 5、12、20 and 30 days after the operation. The results demonstrated that labyrinthectomy increased GAP 43 mRNA levels. The application of ISHM laid a foundation for the research of regenerative sprouting, synaptic remodeling and neuroplasticity in the vestibular compensation.