人血浆HDL对动脉粥样硬化家兔肝细胞膜LDL受体活性影响的研究

Effect of Human Plasma HDL on LDL Receptors of Atherosclerosis Rabbit Liver Plasma Membranes.

高胆固醇饲料喂养造成的动脉粥样硬化（Ａｓ） 模型家兔通过静脉注射人血浆ＨＤＬ 制剂， 观察ＨＤＬ 对Ａｓ家兔肝细胞膜ＬＤＬ受体活性的影响． 结果发现， 摄取高胆固醇饲料的Ａｓ 家兔， 其肝细胞膜ＬＤＬ 受体 Ｋｄ 值虽无明显变化但Ｂｍａｘ 值显著减小（ Ｐ＜ ０－０１ ， 与正常对照组比较） ； 注射ＨＤＬ 制剂后， Ａｓ 家兔肝细胞膜ＬＤＬ受体Ｋｄ 值仍无明显改变， 但Ｂｍａｘ 值却显著回升（ Ｐ＜ ０－０１ ， 与高脂组比较） ． 表明人血浆ＨＤＬ 具有增加Ａｓ 家兔肝细胞膜ＬＤＬ 受体活性的作用．

Atherosclerosis(As)rabbit model was developed by feeding with high cholesterol diet for 12 weeks,then As rabbits were injected intravenously with human plasma HDL preparation for 10 weeks,the effect of HDL on activity of LDL receptors on As rabbit liver plasma membranes was investigated. It was shown that the value of B max of LDL receptor was significantly lower in As rabbit than that in normal rabbit( P <0 01), while the value of K d showed no difference; in HDL treated rabbits, the value of B max increased significantly compared with the rabbits without HDL treatment( P <0 01), while the value of K d showed no difference yet. The results suggested that human plasma HDL could enhance the activity of LDL receptors on liver plasma membranes of As rabbit.