SHR与WKY大鼠肾脏组织基因表达差异分析

Differential Display Analysis of Gene Expression in Kidneys of SHR and WKY Rats

为了观察ＳＨＲ与ＷＫＹ大鼠肾脏组织的基因表达的差异， 用Ｔ１１Ｇ、ＡＰ６ 引物对１２ 周龄的ＳＨＲ 与ＷＫＹ大鼠肾脏ｍＲＮＡ进行了差异显示， 得到了一条在ＳＨＲ大鼠肾脏组织中高表达的差异显示条带， 进一步分析证明这一差异显示条带含有１７０ ｂｐ， 与ＧｅｎＢａｎｋ 收录的已知的核苷酸序列的同源性低于８０ ％ ， 可能为未知基因的ｃＤＮＡ片段， 并定位于ＳＨＲ大鼠的肾小管上皮细胞内．

In order to observe the difference in gene expression in the kidney of SHR and WKY rats, mRNA differential display was undertaken to screen differently expressed genes in the kidney of SHR and WKY rats aged 12 weeks by using T11G and AP6 primer. One cDNA fragment was identified in the kidney of SHR and was selected as interesting band. The techniques of T A cloning, Northern hybridization, in situ hybridization and sequencing of the recombinant plasmid were used to determine the characteristics of differentially displayed cDNA fragment. The results indicate that it contains 170 base pairs and the incidence of homology with known genes is less than 80%, it was located in the renal tubule of SHR and no positive signal was found in the kidney of WKY.