摘要
研究了牛眼透明质酸(HA) 的提纯方法, 采用0-125 mol/LNa2SO4 取代0-1 mol/LNaCl 溶解粗品, 发现前者溶解液中有2 个组分, 一个组分被十六烷基溴代吡啶(CBP) 吸附回收, 产物呈纤维状, 另一组分保留在溶液中并能通过分级稀释而被沉淀回收, 产物呈粉末状; 而用0-1 mol/ml NaCl 溶解粗品, 除了被CBP吸附回收外, 保留在溶液中的HA 不能被回收; 与同类方法相比, 回收率提高1-7 倍以上. 还分析了产物的性质, 与同类样品比较, 提高了纯度, 降低了提纯中有机溶剂的消耗.
A method for purifying HA from ox eyes was studied.Using 0 125 mol/L Na 2SO 4 other than 0 1 mol/L NaCl to dissolve the semifinished product, Two HA forms in Na 2SO 4s solution were found. One in staple could be absorbed by CBP, and the other in powder could be returned by fractional dilution afterwards. In 0 1 mol/L NaCl solution, HA could only be recovered by CBP absorption. It displayed staple. The recoverable rate of the method was 1 7 times than others.The physical and chemical characters of the product were analysed. It was proved that higher purity recovery and lower cost than other methods were gained.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第6期596-600,共5页
Progress In Biochemistry and Biophysics
基金
安徽省重点科研项目
关键词
牛眼
透明质酸
分离
理化性质
提纯
工艺流程
ox eye, hyaluronic acid, fractionation physical and chemical character, quality