牛眼透明质酸的分离及性质测定

A Study on the Separation and Character of Hyaluronic Acid from Ox Eyes

研究了牛眼透明质酸（ＨＡ） 的提纯方法， 采用０－１２５ ｍｏｌ／ＬＮａ２ＳＯ４ 取代０－１ ｍｏｌ／ＬＮａＣｌ 溶解粗品， 发现前者溶解液中有２ 个组分， 一个组分被十六烷基溴代吡啶（ＣＢＰ） 吸附回收， 产物呈纤维状， 另一组分保留在溶液中并能通过分级稀释而被沉淀回收， 产物呈粉末状； 而用０－１ ｍｏｌ／ｍｌ ＮａＣｌ 溶解粗品， 除了被ＣＢＰ吸附回收外， 保留在溶液中的ＨＡ 不能被回收； 与同类方法相比， 回收率提高１－７ 倍以上． 还分析了产物的性质， 与同类样品比较， 提高了纯度， 降低了提纯中有机溶剂的消耗．

A method for purifying HA from ox eyes was studied.Using 0 125 mol/L Na 2SO 4 other than 0 1 mol/L NaCl to dissolve the semifinished product, Two HA forms in Na 2SO 4s solution were found. One in staple could be absorbed by CBP, and the other in powder could be returned by fractional dilution afterwards. In 0 1 mol/L NaCl solution, HA could only be recovered by CBP absorption. It displayed staple. The recoverable rate of the method was 1 7 times than others.The physical and chemical characters of the product were analysed. It was proved that higher purity recovery and lower cost than other methods were gained.