氧化修饰HDL剌激培养人主动脉平滑肌细胞增殖

Effect of Oxidized High Density Lipoproteins on Proliferation of Cultured Human Arterial Smooth Muscle Cells

平滑肌细胞（ｓｍｏｏｔｈ ｍｕｓｃｌｅ ｃｅｌｌ，ＳＭＣ） 增殖在动脉粥样硬化（ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ， ＡＳ） 形成中起着重要作用．氧化修饰ＨＤＬ （ｏｘｉｄｉｚｅｄ ＨＤＬ， ＯＸＨＤＬ） 可刺激３ ＨＴｄＲ 掺入培养人动脉ＳＭＣ 的ＤＮＡ， 促进ＳＭＣ 增殖． 以四甲基偶氮唑盐（ ＭＴＴ） 法直接观察ＯＸＨＤＬ 对培养人动脉ＳＭＣ 增殖细胞数的影响． 结果显示， 天然ＨＤＬ（ｎａｔｉｖｅ ＨＤＬＮＨＤＬ） 对ＳＭＣ增殖没有影响， 而ＯＸＨＤＬ 则显著刺激ＳＭＣ 增殖（ Ｐ＜ ０－０１） ， ＮＨＤＬ 显著抑制ＯＸＬＤＬ 刺激ＳＭＣ增殖作用（ Ｐ＜ ０－０１） ， 而ＯＸＨＤＬ则显著增加ＯＸＬＤＬ刺激ＳＭＣ 增殖作用（ Ｐ＜ ０－０１）

The proliferation of arterial SMC plays an important role in process of atherosclerosis. Oxidized HDL(OX HDL) can increase 3H TdR incorporation to DNA of cultured human arterial SMC, so promote proliferation of SMC. A study on effect of OX HDL on proliferation of cultured human arterial SMC with MTT method was made. It was showed that native HDL(N HDL) has no effect on proliferation of SMC but OX HDL significantly stimulated the proliferation of SMC ( P <0 01);N HDL significantly inhibited proliferation of human SMC induced by OX LDL ( P <0 01) and OX HDL significantly enhanced proliferation of human SMC induced by OX LDL ( P <0 01).