环境温度条件对口蘑菌L1纤维素酶和木聚糖酶合成的影响

Effect of environmental temperature on formation of cellulase and xylanase in Thricholomataceae L1

较高的环境温度有利于口蘑菌Ｌ１ 的生长和蛋白质的合成．当环境温度为３８ ℃时，纤维素酶的比活力达最高值，为２３－４Ｉｕ·ｍｇ－ １ 蛋白质，该菌在此温度下培养９０ｈ ，纤维素酶活力为３３Ｉｕ·ｇ－ １ 干培养基．木聚糖酶的最适合成温度为３２ ℃，此温度下木聚糖酶的比活力可达９２Ｉｕ·ｍｇ － １ 蛋白质，培养６７ｈ ，木聚糖酶活力可达１２６Ｉｕ·ｇ－ １ 干培养基．提示环境温度对口蘑菌Ｌ１ 细胞内酶的合成具有调节作用．

Higher environmental temperature is beneficial to the growth of Tricholomataceae L1 and its protein synthesis. When temperature is 38℃, the specific activity of cellulase is the highest, reaching 23.4Iu·mg -1 protein. After cultivated for 93 hours at this temperature, its cellulase activity is 33Iu·g -1 dry medium. The optimal temperature for the formation of xylanase is 32℃. Under this temperatrue, the specific activity of xylanase is 92Iu·mg -1 protein, and after cultivated for 67 hours, it is 126Iu·g -1 dry medium. It is suggested that environmental temperature has a regulation effect on the formation of intracellular enzyme of Tricholomataceae L1.