Isoverbascoside对HL-60细胞的诱导分化和细胞毒作用

INDUCED-DIFFERENTIATION AND CYTOTOXICITY OF ISOVERBASCOSIDE ON HL-60 CELLS

不同时间、不同浓度的isoverbascoside体外处理HL-60细胞,以形态改变(光镜和透射电镜观察)、功能分化(化学发光检测吞噬能力)、恶性度降低(裸鼠成瘤试验)等指标观察其诱导分化作用;以台盼蓝拒染作用和电镜下形态变化确定其细胞毒作用;用流式细胞术测定其对HL-60细胞周期的影响。20—25μmol/L Isov 1—3天诱导HL-60细胞向粒系方向分化,细胞吞噬能力提高,裸鼠成瘤性降低。30—35μmol/L Isov 2—3天对HL-60细胞有强烈的细胞毒作用。20μmol/L Isov处理12h可引起HL-60细胞的G_1期阻滞,在72h时引起HL-60细胞的G_2/M期的阻滞。

HL-60 cells were treated by isoverbascoside with different time and different concentrations in vitro . The differentiation of HL-60 cells was evaluated by light and electron microscopy to observe morphological changes , by chemilumi-nence to detect phagocytosis and by tumorigene-sis in nude mice to determine malignancy . The cytotoxical effect of isoverbascoside on HL-60 cells was examined by trypan blue excluding staining and electron microscopy. The influence of isoverbascoside on cell cycle was measured by flow cytometry. Granular differentiation of HL-60 cells was induced by isoverbascoside at 20-25μmol/L within 1-3 days as the results of morphological changes , enhancement of phagocytosis and decreasing of tumorigenesis . Strong cy-totoxicity was evidenced in HL-60 cells treated by isoverbascoside at 30-35μmol/L. HL-60 cells treated by isoverbascoside at 20μmol/L were delayed at G1 phase at 12 hours and G2/M phase at 72 hours.