凋亡相关分子PF18-3在ConA诱导活化后胸腺细胞凋亡中的表达特性分析

CHARACTERIZATION OF EXPRESSION OF THE APOPTOSIS ASSOCIATED PF18-3 MOLECULE IN THYMOCYTE APOPTOSIS FOLLOWING ConA ACTIVATION

对抗体PF18-3识别分子(PF18-3分子)在胸腺细胞活化后凋亡过程中的表达特性进行了观察分析,结果表明:经ConA活化后胸腺细胞经历活化后凋亡,表现为DNA梯状片段产生的时相后移,活化后期细胞TUNEL染色阳性。经FACS门技术分析确认,PF18-3分子在亚二倍体高含量的凋亡胸腺细胞亚群特异表达。与凋亡相关的Fas和膜磷脂易位表达的动态比较提示,PF18-3分子与前两类分子不同,可能为新型胸腺细胞凋亡相关分子。

The expression pattern of McAb PF18-3 recognized molecule in activation induced thymo-cyte apoptosis was analysed. Results indicated that following activation by ConA > thymocytes underwent activation induced apoptosis identified by delayed production of DNA ladder and TUNEL positive staining of thymocytes in late stage of activation. PF18-3 molecule was found to express specifically in subset of apoptotic thymocytes with diploid. Kinetic comparison of expression between PF18-3 molecule and Fas or translocated membrane phospholipid suggested that PF18-3 molecule is different from them and likely to be a novel molecule related to thymocyte apoptosis.