培养基初始pH值对木聚糖酶合成的影响

Effects of initial pH value of medium on xylanase synthesis by Trichoderma reesei

本文探讨了培养基三种初始pH值对木聚糖酶合成的影响。试验结果表明，培养基初始pH值对木聚糖酶的合成有重大影响，低pH有利于提高木聚糖酶活力。在碳源为7g／L条件下，初始pH值为4．0时合成的木聚糖酶活力高达32．87IU／ml，酶产率和得率分别为6574．0IU／L·d和4695．7IU／g木聚糖。酶活力和酶产率均是初始pH5．0的1．7倍，分别是初始pH6．0的3．8倍和2．3倍。进一步研究表明，初始pH4．0更有利于提高β-木糖旮酶的活力。

Initial pH value of medium had an important influence on xylanase synthesis by Trichoderma reesei Rut C30. The results showed that low pH value was beneficial to increasing the activity of xylanase. Among three initial pHvalues of media to induce xylanase, initial pH value 4. 0 was optimal , initial pH value 5. 0 was secondary and initial pHvalue 6. 0 was poor . When carbon source was 7 g/L and initial pH value was 4. 0, the activity of xylanase was up to32. 87 IU/ml, with the productivity and yield being 6574. 0 IU/(L. d) and 4695. 7 IU/g xylan, respectively . Furtherstudy indicated that initial pH value of medium was more beneficial to increasing β-xylosidase activity. This means theinfluence of pH on the synthesis of β-xylosidase was greater than that on the synthesis of endo-xylanase .