胃粘膜损伤中EB病毒感染与P53抑癌基因突变的研究

EB Virus Infection and Mutation of p53 Suppressor Gene in Gastric Mucoma Damages

为了探讨EB病毒（EBV）感染与胃癌发生的关系，应用PCR技术对166例胃粘膜损伤标本的EBVDNA进行检测．其中66例应用银染PCR－SSCP分析技术检测了p53exon5～8突变情况，10例应用直接法原位PCR技术检测了EBV在组织中的感染情况。结果166例标本中EBV感染率为30．1％；EBV在细胞中感染大体是弥散型，主要存在于细胞核。66例标本中p53基因突变率为54．5％。对比分析，EBV阳性标本中p53基因突变率为75％（21／28），EBV阴性标本中p53基因突变率为39．5％（15／38）。结果表明，EBV对胃粘膜组织细胞具有易感性，p53基因突变在胃粘膜病变中是一个常发事件，EBV感染与p53基因突变之间存在着高度相关性．对癌症的发生具有重要作用。

To investigate the relationship between EBV infection and genesis of gastric cancer,PCR, PCR-SSCP assay and direct in situ PCR technique were performed on samples of gastricmucoma damages; EBV infection rate was 30. 1 % in 166 samples; EBV was scattered in tissuesand roughly in nucleuses. p53 gene mutation rate was 54. 5% in 66 samples. In contrast, p53gene mutation rate was 75% in EBV-positive samples (21/28), but 39. 5 % in EBV-negative ones(15/38). The results suggest that EBV is infectious for gastric mucoma cells, p53 gene mutationis common in gastric mucoma pathological changes, EBV infection has high correlation with p53mutation, and is of importance during the genesis of cancer.