人类疱疹病毒7型感染脐血单个核细胞产生TNF-α

Human Herpesvirus 7 Induces Tumor Necrosis Factor Alpha in Umbilical Cord Blood Mononuclear Cell Cultures

用生物活性法，检测人类疱疹病毒7型Glasgow株和南京地方株YY5及HHV-6GS株感染单个核细胞培养上清中肿瘤坏死因子－α（TNF-α）的水平。结果发现，HHV-7对也能较强地诱生TNF-α，但达到峰值时间（3～4d）迟于HHV-6GS株（2d）；在感染24h上请中，GS株产生TNF-α量明显多于YY5株、Glasgow株产生量（P＜0．05），三者与未感染单个核细胞比较都存在显著差异（P＜0．05），但Glasgow珠和YY5株之间无差异（P＞0．05）。结果表明，HHV-6、HHV-7都能通过刺激单个核细胞产生TNF-α而发挥免疫调节功能。

The production of tumor necrosis factor alpha (TNF-Q) induced by human herpesvirus7 (HHV-7) Glasgow strain and Nanjing local strain YY5 and HHV-6 GS strain in umbilical cordand pheripheral blood mononuclear cells cultures was studied by means of biological activity assay.It was observed that HHV-7 is another strong inducer of TNFQ. HHV-7-infected culturesreached the maximal level of TNF-α at 3 to 4 days postinfection, which is later than that of HHV6 (2 days Postinfection). Compared with HHV-6, the amounts of TNF-Q in HHV-7-infected cultures are smaller at 24 h postinfection (P<0. 05), but the amounts of them are all higher thanthat of uninfected cells (P<0.05), and there is no difference between Glasgow and YY5 strain(P>0.05). These results indicated that HHV-6 and HHV-7 can exert immunomodulatory bystimulating the CBMCs to produce TNF-α.