对虾白斑综合征杆状病毒体内增殖模型的建立

Establishment of a Model for Proliferating White Spot Syndrome Virus in vivo

应用对虾白斑综合征杆状病毒（WSSV），对淡水克氏螯虾、罗氏沼虾、日本沼虾和两种淡水蟹（中华绒螯蟹、长江华溪蟹）进行人工感染实验。结果除淡水克氏螯虾之外，其它受试的虾蟹均不能感染WSSV。克氏螯虾3个不同剂量级感染至12d平均死亡率为94％。从发病或死亡个体采集血淋巴，经电镜负染色可观察到完整的病毒粒子，其形态大小、靶细胞组织病理均与从中国对虾中分离的WSSV相似或相同。同时，通过原位杂交技术进一步证明该实验的可靠性。克氏螯虾重复感染效果良好，有可能成为研究WSSV的一种理想的病毒体内增殖模型。

With white spot syndrome virus (WSSV) isolated from disease shrimp Penaeus chinensis, some of freashwater prawn and crabs: crayfish Cambarus clarkii, Macrobrachium rosenbergii, Macrobrachium nipponensis,Eriochier sinensis, Sinopotamon yangtsekiense were selected to be artificially infected. The result shows that only Cambarus clarkii can be infected byWSSV. The average mortality of three different dose groups (Group 1, 2, 3) was 94 % during 12days after injection. Intact viral particles can be seen from the hemolymph of dead or diseasedcrayfish using TEM negative staining, the shape of virion and their morphogenesis in host cell arequite similar or identical to the WSSV isolate. Hybridization in situ technique was used to confirm the reliability of the experiment. The result can be repeated using diseased Cambarus clarkiito infect the same kind of crayfish, it suggests crayfish Cambarus clarkii can be used as a goodmodel for the further study of WSSV.