豚鼠气管平滑肌细胞培养

Primary Culture of Tracheal Smooth Muscle Cells of Guinea Pigs

目的建立一种简便、快捷的豚鼠气管平滑肌细胞培养方法。方法幼年豚鼠气管平滑肌组织块原代培养法。结果豚鼠气管平滑肌组织块贴壁5～8 d后可见细胞自组织块周围长出,细胞长梭形,核位于中央,呈辐射状向四周生长。两周后细胞长满瓶壁。经α-平滑肌肌动蛋白单克隆抗体(α-smooth muscle actin monoclonal antibody,α-SMA)免疫组化染色鉴定,获得的细胞为平滑肌细胞。结论豚鼠气管平滑肌组织块培养法可快速获得数量充足的气管平滑肌细胞。

Objective To establish a method of primary culture tracheal smooth muscle（TSM） cells of guinea pigs.Methods Cultured TSM cells of guinea pigs by tissue cultivation and identified TSM cells by the method of immunocytochemistry stain with specific α-smooth muscle actin antibody.Results Cells in fusiform or polygon shape erupted around the pieces of smooth muscle tissue and grew and proliferated radiat with a nucleus lying in the middle of cytoplasm in 5~8 days.The immunocytochemical stain of the monoclonal antibody of anti-smooth muscle α-actin was positive and the purity of the cells was above 95%,which indicated that most of cultured cells were smooth muscle cells.Conclusions The method of culture TSM cells of guinea pig is successful and can be applied.