顺铂致小型猪急性肾衰模型

Established Acute Renal Failure Model in Guizhou Miniature Pigs

目的建立顺铂诱导的贵州小型猪急性肾功能衰竭模型。方法将贵州小型猪随机分为对照组和模型组。以4 mg/kg顺铂一次性腹腔注射,膀胱插管收集尿液,动态观察顺铂处理前后第0、1、3、6、24、48、96 h时段小型猪的尿量,检测尿中肌酐及尿素氮浓度,计算各时段肌酐和尿素氮的排出量。结果与对照组比较,模型组尿量和尿中肌酐、尿素氮的排出量显著减少。结论经顺铂4 mg/kg处理能复制出典型的贵州小型猪急性肾功能衰竭模型。

Objective To establish an acute renal failure model induced by cisplatin in Guizhou miniature pigs.Methods Guizhou miniature pigs were randomly divided into the control group and the model group.Animals of the model group were given cisplatin（4 mg/kg） by a single peritoneal injection.Urine samples were collected by a bladder fistula.Urinary output,urine level of creatinine and urea nitrogen were dynamically observed on the 1st hour before injection and the 1st,3rd,6th,24th,48th,96th hours after cisplatin treatment.The excretions of creatinine and urea nitrogen were calculated on the basis of the above determination.Results Urinary output,excretion of creatinine and urea nitrogen of the model group were significantly lower than that of the control group.Conclusions This study established successfully the acute renal failure model induced by cisplatin（4 mg/kg） in Guizhou miniature pigs.