摘要
成团泛菌工程菌308R(pCPP430)带有梨火疫欧文氏杆菌的与过敏反应和致病性有关的基因簇 (hrp),可以产生能诱导植物抗病性的蛋白质harpin。该工程菌在LB液体培养基中生长50代后,带有重 组质粒pCPP430的细胞占总菌量的 1%,带有载体pCPP9的细胞为46%。工程菌喷雾到番茄叶面,保湿条 件下叶面菌量维持在 105cfu/cm2以上,其中带有 pCPP430质粒的菌维持在 40%以上,带有 pCPP9载体的 菌维持在80%以上。因此,携带hrp基因簇的质粒pCPP430在宿主菌中是不稳定的。文中讨论了改进工 程菌遗传稳定性的途径。
The genetic stability of Pantoea agglomerans 308R(pCPP430), a recombinant strain capable of producing the plant resistance inducing protein harpin, was studied both in medium and in plant under antibiotic-free conditions. After successive growth in LB medium for 50 generations, only 1% of the cells still retained the plasmid pCPP430, in comparison with 46% of the vector pCPP9. After sprayed onto the leaves of tomato, the recombinant strain maintained a population density of 10~5cfu /cm2 when kept under high humidity. 40% cells of this population contained the plasmid pCPP430. It was shown that plasmid pCPP430 was unstable due to inadequate partition. Strategies for improving its genetic stability were discussed.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第6期400-403,共4页
Microbiology China
基金
国家高技术研究发展计划项目("863"项目)
Project of Chinese National Programs for
关键词
工程菌
质粒
遗传稳定性
诱导抗性
成团泛菌
Recombinant bacteria, Plasmid, Genetic stability, Resistance inducing, Pantoea agglomerans