康宁木霉液体深层发酵生产纤维素酶

PRODUCTION OF CELLULASE VIA SUBMERGED FERMENATION OF TRICHODERMA KONINGⅡ

以康宁木霉（ Ｔｒｉｃｈｏｄｅｒｍａ ｋｏｎｉｎｇｉｉ） Ｔ２１５为生产菌，在 ３０ｔ气升式发酵罐中进行了液体深层发酵生产纤维素酶的扩大试验。一、二级罐种子培养基由８％麦麸组成，种龄２４ｈ。产酶培养基由６％稻草粉，１％麦麸和１％蛋白胨组成，起始ｐＨ５．０。每罐装２２ｔ培养基，１０％接种量，通气量０．４ｖｖｍ，罐压０．０８ＭＰａ，２９℃±１℃培养 ９６ｈ。连续试验 ５批，平均发酵液酶活力： ＣＭＣ－Ｎａ活力 ７８．３ＩＵ／ｍＬ，脱脂棉活力 １.３ＩＵ／ｍＬ，水杨苷活力１.４ＩＵ／ｍＬ，滤纸活力５．４ＩＵ／ｍＬ。

An experiment for cellulase production was carried out in a 30t air left fermentor with an operating volume of 22t. Trichoderma koningii T215 growing in the liquid medium which consisted of 6% rice straw powder, 1% wheat bran and 1% peptone (initial Pp5.0). Operating conditions: seed, 10% inoculum of a 24h old fermentor culture growing in a fermentor in the medium consisted of 8% wheat bran, aeration, 0.4vvm; press, 0.08MPa; temperature, 29℃±1℃; Period, 96h. Cellulase activity of the supernatant of the culture as follows: CMC-Na activity 78.3 IU / mL, absorbent cotton activity 1.3 IU / mL, salicin activity 1.4 IU / mL, filter paper activity 5.4 IU/mL.