HAT-RAPD技术在姜科象牙参属植物自然杂交个体鉴定中的应用

Application of HAT-RAPD Technique in Identifying Natural Hybrids of Roscoea(Zingiberaceae)

自然杂交在植物中广泛存在,对植物进化起着重要的作用。研究自然杂交现象,除了传粉、形态等传统手段,还需要分子水平的证据。本研究利用HAT-RAPD技术,对姜科象牙参属植物的自然杂交现象进行分析,运用主坐标分析和杂交指数分析对疑似杂交个体进行鉴定,探讨HAT-RAPD技术在分析和鉴定杂交个体方面的可行性。结果显示,HAT-RAPD方法比传统RAPD扩增的条带更稳定、清楚、容易统计,同时主坐标分析和杂交指数分析的结果表明,形态介于大花象牙参(Roscoea humeana)和早花象牙参(R.cautleoides)的个体是两者的杂交后代,且与大花象牙参的亲缘关系更近。以上的结果表明HAT-RAPD技术可以用于自然杂交的分析,并且由于其简单、易操作等特点,将成为一种非常适合分析和鉴定杂交个体的分子手段。

Natural hybridization is very common in plants, and plays an important role in plant evolution. Besides the traditional methods including morphological analysis and hand crossing, molecular evidence is needed for study- ing natural hybridization. In order to analyze natural hybridization in Roscoea, HAT-RAPD technique was used to identify putative hybrids from parental species by principal co-ordinate analysis and hybrid index. The results indica- ted that the bands amplified by HAT-RAPD technique were more stable and reliable than that of RAPD. The result of principal co-ordinate analysis and hybrid index showed that intermediate individuals were the hybrids of R. hu- rneana and R. cautleoides, and showed closer relationships to R. humeana. These results suggested that HAT-RAPD could be used to study natural hybridization. As it is simple and easy to manipulate, HAT-RAPD may prove to be a very effective technique in hybrid identification in the studies of plant evolution.