沙田柚花粉发育过程中同工酶的变化研究

Study on varieties of isozymes during deve lopment of pollen of Citrus grandis var-shatinyu Hort

分析了沙田柚花粉在发育过程中３ 种酶的同工酶的活力及类型的变化。结果表明， 过氧化物酶活力在单核期达到最大， 在四分体期与单核期所具有的一条染色较深的快速酶带在双核期消失。α－ 淀粉酶活力在成熟期达到最大， 谱带上也表现出在成熟期有酶带的增多。分析酯酶酶谱， 可见其在各个发育时期均有明显的特征带， 这些特征带可作为花粉发育各时期的生化指标。

The varieties of activity and type of three isozymes of shatinyu pollen during the pollen development were analyzed The results show the activity of peroxidase reach the maximum in its mononuclear stage, A dark dyeing and fast speed enzyme band in the tetrad stage and mononuclear stage disappeared at binuclear stage α-amylase activity reached maximum during the mature stage The diagram indicated the bands increased at mature We discovered esterase had an obvious character band at the different phase of development of pollen, which can be served as biochemical symbol of the different development phase of shatinyu pollen