氯霉素残留胶体金免疫层析快速检测法的研制

Development of Colloidal Gold Immunochromatographic Assay for Rapid Detection of Chloramphenicol Residue

建立一种快速、简易的检测样品中氯霉素残留含量的胶体金免疫层析法.将抗氯霉素单克隆抗体-胶体金复合物包被在胶体金结合垫上,并将人工合成的氯霉素抗原包被在硝酸-醋酸纤维素薄膜表面作为检测线(T线),其与待测样品中氯霉素竞争结合胶体金标记的氯霉素单克隆抗体,并能以颜色直观显示检测的结果.用GICA与ELISA试剂盒对比检测了60份牛奶标本,两法符合率为100%.GICA检测样品中氯霉素残留含量异性强,灵敏度高,简便快速,无需特殊仪器设备.

To establish a rapid, simple colloidal gold immunochromatographic assay（GICA） for the detection of chloramphenicol （CAP）residues. Nanocolloidal gold particles were prepared and labeled to an anti-CAP monoc- lonal antibody （MAb）. CAP was conjugated to bovine serum albumin （BSA） and dispersed on a nitrocellulose （NC） membrane to be the test line （T）. The more analyte present in the sample, the more effectively it would compete with the CAP-BSA for binding to the limited amount of gold labeled anti-CAP MAb. The presence or absence of a colored band on the test line indicates a negative or positive result. A total of 60 milk samples were detected for CAP by GICA and ELISA. The corresponding rate of both tests was 100 %. GICA is a sensitive, specific, simple and rapid assay for detecting CAP in the sample.