基因组原位杂交鉴定玉米的外源染色体片段

Identification of Zea diploperennis Chromosome Fragments Introgressed to Maize via Genomic in Situ Hybridization

利用基因组原位杂交技术 ,在稳定遗传的纯系 5 40及其与玉米 (ZeamaysL .)自交系杂交后代F1,即遗单 6号中 ,成功地鉴定了渗入其中的二倍体多年生类玉米 (ZeadiploperennisDoebley ,DP)的染色体片段。采用DAB (Di aminobenzidinetetrahydrochloride)和荧光检出系统 ,二者获得了一致的结果。在纯系 5 40的第 1、2、5和 8号两条同源染色体长臂上均检出杂交信号 ,而仅在遗单 6号第 1、2和 8号染色体长臂上具有信号点。在纯系 5 40和遗单 6号中 ,第 1和 2号染色体上的信号点距着丝粒的百分距离十分一致。遗单 6号的第 8号染色体上的信号点距着丝粒的百分距离比纯系 5 40的小 ,并讨论了产生这种差异性的原因。

ZeadiploperennisDoebley (DP)chromosomefragmentsintrogressedtomaize (ZeamaysL .)were identifiedbygenomicinsituhybridizationinthestablealloplasmicpureline 540anditshybridF1,Yidan 6 wasobtainedbycrossingwithmaizeinbredline .Diaminobenzidinetetrahydrochloride (DAB)andfluorescence stainingsystemswereutilizedfordetectionofthehybridizationsignalsrespectively ,andbothofthemgaveal mostthesameresults .ThehybridizationsignalsofDPchromatinwereshowedonthelongarmsoftwomembers foreachofchromosomes 1,2 ,5,and 8inpureline 540andonthoseofonlyonememberforeachofchromo somes 1,2 ,and 8inYidan 6 .NotonlylocatedDPchromatinonthesamechromosomearmsbutalsotheir percentagedistancesfromthecentromerestothehybridizationsiteswereclosetoeachotherforchromosomes 1 and 2betweenpureline 540andYidan 6 .Thepercentagedistanceofthesignalonthelongarmofchromo some 8wasnotablyshorterinYidan 6thanin 540 .Thedifferencesofthesignaldistributionbetweenpureline 540andYidan 6werediscussed .