摘要
A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes (MMCNT) which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope (SEM) and vibrating sample magnetometer (VSM) were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica (CS) hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10-7 to 0.165×10-3 mol/L. The corresponding detection limit is 3.34×10-8 mol/L based on signal-to-noise ratios (S/N) ≥3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.
A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes （MMCNT） which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope （SEM） and vibrating sample magnetometer （VSM） were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica （CS） hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10-7 to 0.165×10-3 mol/L. The corresponding detection limit is 3.34× 10-8 mol/L based on signal-to-noise ratios （S/N） ≥3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.
基金
Project(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University, China
Projects (50978088, 51039001) supported by the National Natural Science Foundation of China
Project(2009FJ1010) supported by the Hunan Key Scientific Research Program, China
Project(10JJ7005) supported by the Natural Science Foundation of Hunan Province, China
Projects(CX2009B080, CX2010B157) supported by the Hunan Provincial Innovation Foundation For Postgraduate
Project supported by the Fundamental Research Funds for the Central Universities, Hunan University, China
