摘要
利用蚕豆根尖细胞微核测定技术对垃圾渗滤液的微核效应进行了监测．结果表明，填埋场垃圾的微核率为１２．７０×１０－３ ，渗滤液的微核率为（１６ ．７６ ～２９．５）×１０ －３ ．垃圾场底部０～１０ ｃｍ 处的土层已被污染，微核率为１５．３８ ×１０－３ ，与ＣＫ有显著性差异；而３０ ～４０ ｃｍ 处土层的微核率为７ ．６５ ×１０－３ ，与ＣＫ无显著性差异，提示该处土层对垃圾渗滤液中污染物向下迁移有拦截、净化作用．经过处理后的渗滤液的微核率明显降低．蚕豆根尖微核检测结果与化学分析结果相吻合，认为此法可用于对垃圾渗滤液污染状况进行生物监测．
The micronucleus test (MCNT)on root tip cells of Vicia faba was used to monitor the micronucleus effects of refuse leachate. The results indicated the micronucleus rate of refuse in landfill was 12.7 ×10 -3 and the micronucleus rate of the leachate was up to (16.70～29.50)×10 -3 . In the landfill the bottom soil of 0～10 cm was polluted, the micronucleus rate was 15.38×10 -3 and greatly different from CK.However,the micronucleus rate in the soil of 30～40 cm deep was 7.65×10 -3 and not greatlydifferent from CK ,indicating that soil could intercept and purify refuse leachate.After treatment,the micronucleus rate of leachate was greatly reduced.MCNT had the same results as those made by chemical analyses and could be used to monitor the pollution of refuse leachate.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第6期574-577,共4页
Chinese Journal of Applied and Environmental Biology
关键词
微核测定
蚕豆根尖
垃圾渗滤液
污染
生物监测
biological monitoring of genetic toxicity
micronucleus test
root tip of Vicia faba
refuse leachate
pollution
