有机磷农药降解菌的紫外诱变育种

BREEDING BY ULTRAVIOLET RAY MUTATON OF ORGANOPHOSPHOROUS PESTICIDES-DEGRADING STRAIN OF BACILLUS

有机磷农药降解菌地衣芽孢杆菌（ Ｂａｃｉｌｌｕｓｌｉｃｈｅｎｉｆｏｒｍｉｓ） 经紫外线诱变后，筛选出突变菌株Ｐ１２ ．在θ＝３０℃，溶解氧ρ（Ｏ２） ＝２ ．５ ｍｇ Ｌ－１ 的培养条件下，３ ｄ 内对甲胺磷的降解率为８０ ．１％ ，比出发菌株提高了将近１０％的降解率．农药斜面连续传代１０ 次，降解活力保持稳定．

A mutant P 12 was obtained from an organophosphorous pesticides-degrading strain( Bacillus licheniformis ) mutated by ultraviolet ray . At θ =30℃, ρ (O 2)=2.5 mg L -1 , the removal rate of methamidophs by the strain was 80.1% in 3 d and was 10% higher than that by original strain. The mutant was generated for ten times on the tube medium containing methamidophs and showed that the degradative capacity was stable.