类风湿关节炎患者红细胞C_(3b)受体数目改变及基因多态性分析

The number of erythrocyte C_(3b) receptor (C_(3b) R) and polymorphism of C_(3b) gene in patients with rheumatoid arthritis (RA)

目的 分析类风湿关节炎（ＲＡ） 患者Ｃ３ｂ受体（Ｃ３ｂＲ） 表达数目及Ｃ３ｂＲ 基因多态性分布规律，探讨Ｃ３ｂＲ 数目改变的可能原因。方法 分别测定６３ 例ＲＡ 患者及健康献血员６０ 人的红细胞Ｃ３ｂ 受体花环率（Ｃ３ｂＲＲ） 及免疫复合物花环率（Ｃ３ｂＩＣＲ） 。用限制性片段长度多态（ＲＦＬＰ） 分析及Ｓｏｕｔｈｅｒｎ ｂｌｏｔｔｉｎｇ 杂交技术检测Ｃ３ｂＲ基因多态性。结果 ＲＡ 患者红细胞Ｃ３ｂＲＲ 和Ｃ３ｂＩＣＲ 分别显著低于和高于正常对照组。Ｃ３ｂＲ基因的两个等位基因基因型和表现型频率在ＲＡ 组和对照组均无显著差异。结论 ＲＡ 患者Ｃ３ｂＲ数目下降可能与基因变异无关，可能是受体基因转录和翻译过程异常所致。设法增加红细胞Ｃ３ｂＲ 数目以加速免疫复合物的清除，可能是治疗ＲＡ的有效途径之一。

Objective To analyse the number of erythrocyte C 3b receptor (C 3b R) and polymorphism of C 3b R gene in patients with rheumatoid arthritis (RA) and to probe into the cause of changes of C 3b R number.Methods The erythrocytes C 3b RR and C 3b ICR in 63 patients with RA and 60 healthy volunteers were detected.Meanwhile,the polymorphism of C 3b R gene were analysed with restriction fragment length polymorphisms (RFLP) and southern blotting technique.Results The numbers of C 3b RR and C 3b ICR were significantly decreased and increased in RA patients than those in healthy volunteers respectively.However,there was no difference in the frequency of genotype and phenotype for the two alleles of C 3b R between RA patients and healthy volunteers.Conclusion The lower number of erythrocyte C 3b R in RA patients may not be caused by the C 3b R gene polymorphism and could be caused by an abnormality of transcription and translation for C 3b R gene.The increase of the number of C 3b R on erythrocyte to accelerate the clearance of immune complex may be a good way for the treatment of RA.