菩人丹超微粉对糖尿病大鼠视网膜碱性成纤维细胞因子表达的影响

Effects of Purendan superfine powder on expression of retinal basic fibroblast growth factor in diabetic rat

目的:探讨菩人丹超微粉对糖尿病大鼠视网膜碱性成纤维细胞因子(bFGF)表达的影响。方法:48只Wistar大鼠随机分为4组,正常对照组、糖尿病模型组(模型组)、菩人丹超微粉治疗组(菩人丹组)和导升明组,每组12只。糖尿病模型组、菩人丹超微粉治疗组和导升明组大鼠均采用链脲佐菌素(STZ)连续腹腔注射建立2型糖尿病大鼠模型。模型成功建立后,菩人丹超微粉治疗组大鼠给予菩人丹超微粉灌胃,导升明组大鼠给予导升明灌胃,时间均为3个月。采用SP免疫组织化学染色法、Western blot法检测视网膜bFGF蛋白的表达;逆转录聚合酶链反应(RT-PCR)检测视网膜bFGF mRNA的表达。结果:糖尿病模型组与正常对照组比较,大鼠视网膜bFGF蛋白及mRNA表达均明显升高(P<0.01),菩人丹超微粉治疗组、导升明组与糖尿病模型组比较,大鼠视网膜bFGF蛋白及mRNA表达均明显降低(P<0.01);且菩人丹超微粉治疗组与导升明组比较无明显差别。结论:菩人丹超微粉可通过下调bFGF表达,抑制病理性新生血管生成,发挥对糖尿病视网膜损伤的保护作用。

Objective： To investigate the effects of Purendan superfine powder（PRD） on retinal basic fibroblast growth factor（bFGF） expression in diabetic rat.Methods： 48 male Wistar rats were randomly divided into 4 groups（n=12）： normal control group,diabetes model group,PRD treatment group and Doxium group.The diabetes model rats were made by continuous intraperitoneal injection of Streptozotocin（STZ）.After the diabetes animal model was successfully established,the rats in PRD treatment group and Doxium group were respectively lavaged with PRD and Doxium for 3 months.Immunohistochemical staining,Western blot were used to assess the expression of bFGF protein in retina;reverse transcription-polymerase chain reaction（RT-PCR） was employed to examine the expression of bFGF mRNA in retina.Results： The expressions of bFGF protein and mRNA in retina were obviously higher in diabetic rats than in normal control rats（P0.01）.The expressions of bFGF protein and mRNA in retina were obviously lower in PRD treatment group and Doxium group than in diabetes model rats（P0.01）.However,there was no obvious difference between PRD treatment group and Doxium group.Conclusion： PRD can decline the expression of bFGF in retina of diabetic rats,so to protect the tissues in diabetic retinopathy.