轮泻停药膏敷脐对小鼠肠推进及腹泻炎性介质的影响

Effects of Lunxieting Ointment pasted umbilicus in intestinal propulsion and diarrhea inflammatory mediators of mice

目的:初步探讨轮泻停药膏敷脐对小鼠肠推进以及对番泻叶所致小鼠腹泻后炎性介质的变化的影响,为临床研究治疗轮状病毒腹泻提供有效依据。方法:将所有实验小鼠随机分为3部分,每部分4组,即空白组、模型组、思密达组及轮泻停药膏组。模型组和空白组给予等量生理盐水,思密达组按(0.06g/mL)0.02mL/g,2次/d灌服,轮泻停药膏组贴敷轮泻停药膏,1剂/d;除空白组,其他组小鼠注射甲硫酸新斯的明,观测小鼠小肠酚红的推进率;观察各组对小鼠给予番泻叶后大便次数增加的变化;检测番泻叶所致小鼠严重腹泻后血清中IL-1β和TNF-α的水平变化。结果:轮泻停药膏组与思密达组比较,小肠的推进距离及推进率差异均有显著性(P<0.05),小鼠大便次数也具有显著性差异(P<0.05);两组的血清IL-1β和TNF-α差异也具有统计学意义(P<0.05)。结论:轮泻停药膏能缓解腹泻程度,保护肠黏膜免受炎性介质的损害,具有潜在的治疗轮状病毒腹泻的疗效。

Objective： To observe intestinal propulsion and diarrhea inflammatory mediators of mice by LunXieting Ointment pasted umbilicus and accordingly to provide experimental basis for clinical treatment of children with rotavirus diarrhea.Methods： neostigmine and senna are used to establish experimental model on mouse with hyperfunction of intestinal motor function and severe diarrhea.Mice were randomLy assigned to four groups.The blank group and the model group were received same volume NS drenched;The LunXieting Ointment group were received LunXieting Ointment pasted Umbilicus after shaving and adhereed with medical adhesive tape;the Smecta group were received Smecta drenched（0.06g/mL） according to 0.02mL/g,two times per day.Subsequently we observed the promoting distance and the rate of promotion on small intestine of mice,the number of diarrheal within 6 hours as well as serum IL-1βand TNF-αlevels.Results： LunXieting Ointment pasted Umbilicus can significantly reduce the hyperactivity of small intestinal motor function,reach a significant reduction in the number of mice with diarrhea and reduce the serum IL-1β and TNF-α levels（P0.01）.Conclusion： LunXieting Ointment can clearly alleviate the diarrhea and protect the intestinal mucosa from inflammatory mediators,which have a potential role in the treatment of rotavirus diarrhea.