博落回提取物(MCE)中主要生物碱与硫酸成盐研究

Salification of main alkaloids in Macleaya cordata extract with sulfuric acid

目的:测定博落回提取物(MCE)中4种生物碱与硫酸成盐的计量关系及其含量,为建立MCE的质量标准提供实验依据。方法:用HPLC法测定其中4种主要生物碱的含量;BaSO4沉淀重量法测定SO42-含量;NaOH酸碱电位滴定法测定H2SO4和HSO4-的总量。结果:硫酸氢根含量约为18%,生物碱的硫酸氢盐含量>80%,游离硫酸含量约为0.76%。结论:MCE中生物碱以硫酸氢盐形式存在,而且血根碱和白屈菜红碱(苯并菲啶类生物碱)为其主要有效成分。

Objective： To determine the metrical relationship and content of four alkaloids in Macleaya cordata extract salification with sulfuric acid,and provide experimental basis for the quality standard ofMacleaya cordata extract.Methods： The content of four kinds of alkaloids were determined by HPLC,and the content of sulfate was determined by barium sulfate sediment weighting method,and the total amout of sulfuric acid and bisulfate ion was determined by sodium hydrate acid base electric titration.Results： The content of bisulfate ion was about 18%,the bisulfate salt of alkaloid was above 80%,and the content of liberated sulfuric acid was 0.76%.Conclusion： The alkaloids in Macleaya cordata extract were existed in the form of bisulfate,and the main active components were sanguinarine and chelerythrine（benzophenanthridine）.