吸烟对外源性弹性蛋白酶诱发的大白鼠肺气肿的影响

A study of the influence of smoking on white rats′ pneumonectasis induced by exogenous proelastase

目的 探讨吸烟在肺气肿发病中的作用。方法 将实验大白鼠分为香烟组、弹性酶组、复合组及对照组四组,分别给予吸烟、气管内注入弹性蛋白酶,7周后测定大白鼠的肺泡平均线性截距(MLI)、平均肺泡数(MAN)、肺置换水容量(TLV)。测定血清弹性蛋白酶抑制力(SEIC)及血清胰蛋白酶抑制力(STIC)。应用方差分析进行统计学处理。结果 形态学变化:肉眼及光镜下可见复合组有明显肺气肿表现,弹性酶组有轻度肺气肿变化,香烟组及对照组无变化。复合组及弹性酶组动物的MLI、MAN有明显变化,P<001,香烟组变化不明显,P>005。血清学结果:各组动物的SEIC及STIC无明显变化,P>005。结论 吸烟增强外源性弹性蛋白酶诱发的大白鼠的肺气肿。

Objective To explore the influence of smoking on the pathogeny of peneumonectasis.Methods Divided the experiment white rats into four groups-smoking group,exogenous proelastase group,combined group and control group.Given the groups smoking,exogenous proelastase injected through tracchea,respectively.Seven weeks later,detected MLI and of these white rats * pulmonary alveoli,TLV,SEIC and STIC,then analyzed the data statistically by analysis of variance.Results ①Morphological change pneumonectasis was noticed under naked eyes and optical microscope in combined;only slight pncumonectasis change could be seen in exogenous proelastase group MLI,MAN in both combined exogenous proelastase group have changed noticeably, P <0.01 While no obvious change happened in smoking group, P >0.05. ②Serolgical results:In the four groups,no great change in SEIC and STIC happened.Conclusion Smoking can increase white rats * pneumonectasis induced by exogeaous proelastase. [