PCNA、CK_(13)、HPV在子宫颈癌的表达

Expressions of PCNA,CK_(13) and HPV in carcinomas of uterine cervix

目的：了解乳头瘤病毒（ＨＰＶ）、细胞角蛋白１３（ＣＫ１３）、增殖细胞核抗原（ＰＣＮＡ） 在子宫颈癌的表达及对子宫颈癌的发生、发展、预后的影响及其相关性。方法：采用免疫组化ＳＰ法和多聚酶链反应ＰＣＲ法，对３０ 例子宫颈癌组织标本石蜡切片中ＨＰＶ、ＰＣＮＡ、ＤＫ１３ 的表达进了观察研究。结果：①感染乳头瘤病毒ＨＰＶ１８型是子宫颈癌的原因之一；②子宫颈鳞癌细胞核中ＰＣＮＡ含量与鳞癌分级呈正相关；③ＣＫ１３ 在子宫颈鳞癌细胞中的表达率可作为判断肿瘤恶性度、预测预后的指标。结论：子宫颈癌的发生与乳头瘤病毒ＨＰＶ１８ 型感染相关；ＰＣＮＡ、ＣＫ１３ 在子宫颈癌的表达可作为判断宫颈癌恶性程度及预后的指标。三者之间尚无明确相关性。

Purpose:To understand the expressions of PCNA,CK 13 and HPV in uterine cervix carcinomas and their correlationships with the occurence,development and prognosis of this tumour.Methods:Expressions of PCNA,CK 13 and HPV of surgical or biopsy specimens taken from 30 uterine cervix carcinomas were determined by immunohistochemical SP method and PCR method. Results:The infective papilloma virus HPV 18 was one of the causes of uterine cervix carcinoma.Content of PCNA in squamous cell carcinoma of uterine cervix was positively correlated with the grading(degree of cell differentiation) of this tumour. The expression rate of CK 13 in squamous cell carcinoma of uterine cervix can be regarded as an index for judging the malignancy and prognosis of this tumour. Conclusion:Occurence of uterine cervix carcinoma is correlated with infective papilloma virus HPV 18 .Expressions of PCNA,CK 13 in this tumour can be regarded as indexes to judge its malignancy and prognosis.There are no definite relations demonstrated among the expressions of PCNA,CK 13 and HPV in carcinoma of uterine cervix.