大豆疫霉拮抗细菌B048液体发酵培养基的筛选与发酵条件的优化

Screening of Liquid Medium and Optimization of Fermentation Conditions for a Bacterium B048 against Phytophthora Sojae

通过单因子实验,筛选得到了适合大豆疫霉拮抗细菌B048液体发酵的最佳碳源为玉米粉,最佳氮源为豆粕,最佳无机盐离子为氯化钠。通过正交实验确定了该培养基的最佳配比为:玉米粉5g/L、豆粕15g/L、氯化钠10g/L。在确定适合该菌株的培养基组分基础上,对其培养条件进行优化,结果表明:初始pH值为6.0,培养时间为48h,转速为180r/min时为该菌株的最佳培养条件。

Single factor test and orthogonal chart were used to optimize the fermentation medium of a bacterium B048. The results indicated that the medium was corn flour 5 g/L, soybean meal 15 g/L, sodium chloride 10 g/L. On the basis of the medium components, the culture conditions were optimized. The re- sults show that the initial pH value is 6, the incubation time is 48 h, and the rotational speed is 180 r/min.