重症急性胰腺炎并肾损害大鼠肾组织Livin、Caspase-9蛋白变化及意义

Changes and significance of Livin,Caspase-9 protein in rats of severe acute pancreatitis associated with renal injury

目的探讨重症急性胰腺炎(SAP)并肾损害大鼠肾组织Livin、Caspase-9蛋白变化及意义。方法将90只大鼠随机分为假手术组(A组)、SAP并肾损害组(SAP组)、褪黑素(MT)干预组(MT组)各30只,观察其胰腺、肾组织病理改变,检测血清淀粉酶(AMY)、肌酐(Cr)、丙二醛(MDA)、超氧化物歧化酶(SOD)活性及肾组织Livin、Caspase-9表达;分析SAP并肾损害与Livin、Caspase-9表达的相关性。结果光镜下,A组胰腺、肾组织均正常;SAP组随造模时间延长病变逐渐加重;MT组病理改变较SAP组减轻。与A组比较,SAP组AMY、Cr、MDA、Caspase-9升高,SOD、Livin降低(P均<0.01);与SAP组比较,MT组AMY、Cr、MDA、Caspase-9降低,SOD、Livin升高(P均<0.05)。SAP组SOD与Cr、Caspase-9表达呈负相关,MDA与Cr、Caspase-9表达呈正相关(P均<0.05);MDA、SOD与Livin表达无相关性(P均>0.05)。结论 Livin、Caspase-9蛋白变化可能参与SAP大鼠的肾损害,外源性MT对SAP并肾损害有保护作用。

Objective To investigate the significant changes of Livin,Caspase-9 protein in rats of severe acute pancreatitis（SAP） associated with renal injury.Methods Ninety SD rats were randomly divided into three groups： the sham operation group（group A）,SAP associated with renal injury group（group SAP） and melatonin intervention group（group MT）,whose pathologic changes of pancreas and kidney were observed.Furthermore,the levels of serum amylase（AMY）,creatinine（Cr）,malondialdehyde（MDA）,superoxide dismutase（SOD） and the expressions of Livin and Caspase-9 in renal tissue were detected.Finally,the relevance between renal injury in SAP and Livin and Caspase-9 was analyzed.Results Pancreas and kidney tissue in group A were observed by light microscopy,and showed no evident lesion;the pathological lesion in group SAP was gradually aggravated with extension of molding time;the pathological lesion in group MT at each time point was more remissive than that in group SAP.Compared with the group A at each time point,expression levels of the serum AMY,Cr,MDA and Caspase-9 in group SAP were increased（P0.01）,and which were decreased in the SOD and Livin（P0.01）;and the results were identical when compared to the group MT（P0.05）.In group SAP,the expression levels of Caspase-9 and Cr were negatively correlated with SOD（P0.05）,and which were positively correlated with MDA（P0.05）;the MDA and SOD had no relevance to Livin respectively（P0.05）.Conclusion The changes of Livin,Caspase-9 protein may be associated with the severity of renal injury of SAP rats,and exogenous melatonin may have a protective effect on renal injury in SAP.