慢性阻塞性肺疾病急性加重期血清MMP-9、TIMP-1变化的临床意义

Changes of serum MMP-9,TIMP-1 in patients with AECOPD and their clinical significance

目的探讨慢性阻塞性肺疾病(COPD)急性加重期血清基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制剂-1(TIMP-1)变化的临床意义。方法选取COPD急性加重期患者55例(COPD组)、查体健康者20例(对照组),根据COPD组肺功能及病情程度分为轻度组15例、中度组22例、重度组18例。采用酶联免疫吸附法检测各组血清MMP-9及TIMP-1;行肺功能通气检查,记录第1秒钟用力呼吸容积(FEV1)/用力肺活量(FVC)及FEV1占预计值的百分比(FEV1%)。分析COPD组血清MMP-9、TIMP-1水平与病情严重程度的相关性。结果 COPD组血清MMP-9、TIMP-1明显高于对照组,且随病情加重逐渐升高(P<0.01或<0.05);直线相关分析显示,COPD组血清MMP-9、TIMP-1水平与FEV1%呈负相关(r分别为-0.783、-0.776,P均<0.01)。结论血清MMP-9、TIMP-1水平与COPD病情程度相关,病情越重,MMP-9、TIMP-1越高,且与FEV1%呈负相关;提示血清MMP-9、TIMP-1变化可作为判定COPD病情严重程度的指标之一。

Objective To investigate the MMP-9,TIMP-1 serum level in patients with AECOPD and their clinical significance.Methods Fifty-five AECOPD patients（COPD group） and twenty healthy controls（control group） were recruited.According to lung function and severity,the AECOPD patients were divided into 3 groups： Mild group（15 cases）,Moderate group（22 cases）,Severe group（18 cases）.Serum levels of MMP-9,TIMP-1 were measured by ELISA.Lung function and FEV1/FVC,FEV1% were tested.Then the correlations between serum levels of MMP-9,TIMP-1 and severity were evaluated.Results The serum levels of MMP-9,TIMP-1 in COPD group were higher than those of control group,and they gradually increased with the severity of COPD（P0.01 or 0.05）.Linear correlation analysis showed that the levels of MMP-9,TIMP-1 negatively correlated with FEV1%（r=-0.783 or-0.776,all P0.01）.Conclusions The serum levels of MMP-9 and TIMP-1 gradually increase with the severity of COPD,and negatively correlated with FEV1%.It indicates that the changes of serum MMP-9 and TIMP-1 may be determined as an indicator of the severity of COPD.