GnRH激动剂对人卵巢癌裸鼠移植瘤抑制作用及不良反应的研究

The inhibition the effect of gonadotropin releasing hormone agonist on the growth of transplanted human epithelium ovarian cancer in nude mice and its side effects

目的研究GnRH激动剂对人卵巢癌裸鼠移植瘤抑制作用及副作用。方法将人卵巢癌细胞OVCAR3注射入裸鼠腹腔内,构建移植瘤模型。将裸鼠随机分为3组,OVCAR3对照组、低剂量曲普瑞林组(75μg)和高剂量曲普瑞林组(150μg)。镜下观察曲普瑞林对全身及腹腔各脏器的毒副作用并测定对内分泌的影响。结果高剂量曲普瑞林组腹腔移植瘤个数和体积最少(P<0.05)。曲普瑞林对脏器无明显不良反应,高剂量曲普瑞林组血清雌激素明显低于OVCAR3对照组和低剂量曲普瑞林组(P<0.05),血清促卵泡素明显高于OVCAR3对照组和低剂量曲普瑞林组(P<0.05)。结论 GnRH激动剂可抑制人卵巢癌裸鼠移植瘤生长,不良反应是引起雌激素缺乏,促卵泡素增多。

Objective To observe the effect of gonadotropin releasing hormone agonist （triptorelin） on the growth of transplanted human epithelium ovarian cancer in nude mice and its side effects. Methods The transplantation tumor models were established by injecting OVCAR3 cells into the peritoneal cavity of nude mice, then the mice were randomly divided into three groups ： control group, low-dose triptorelin group （75μg） and high-dose triptorelin group（150μg）. The effect of triptorelin on endocrine and its side effects on the organs of abdominal cavity and all over the body of the mice were observed under light microscope. Results The high-dose triptorelin could obviously inhibit the growth of transplanted human epithelium ovarian cancer in nude mice, and the number and size of transplantation tumor in abdominal cavity in high-dose triptorelin group were the least among the three groups （ P 〈 0.05 ）. Triptorelin had no obvious side effects on the organs of the nude mice, and the levels of serum estrogens in high-dose triptorelin group were significantly lower than those in control group and low-dose triptorelin group （ P 〈 0.05）, however, the levels of serum follicle stimulating hormone were significantly higher than those in control group and low-dose triptorelin group （ P 〈 0.05）. Conclusion The gonadotropin releasing hormone agonist can inhibit the growth of transplanted human epithelium ovarian cancer in nude mice, with the side effects of lack of estrogen and increase of follicle stimulating hormone.