转基因小鼠肝癌细胞株的建立及生物学特性研究

The construction of transgene mouse hepatoma cell line and study on biological characteristics

目的：将细胞因子基因尽快应用于临床基因治疗。方法：用逆转录病毒载体ｐＬＸＳＮ将小鼠γ干扰素基因（ｍＩＦＮγ）导入小鼠肝癌细胞株，建立了转基因的肝癌细胞株Ｈ２２ｍＩＦＮγ，对该细胞株的生长、形态结构、ＤＮＡ 含量及细胞周期的变化、γ干扰素的分泌、细胞的免疫原性及致瘤性等生物学特性进行了研究。结果：发现该株细胞的生长、形态结构、ＤＮＡ含量及细胞周期均无明显改变，细胞能分泌一定活性的γ干扰素，ＭＨＣＩＩ类抗原的表达量增加，细胞致瘤性明显下降。结论：为进一步开展异体乃至自体瘤苗进行基因治疗创造了条件。

Objective:In ordertoapplycytokinesto clinicalgenetherapyearlier.Methods:Transduced mouseinterferon γ(mIFN γ)gene into mouse hepatomacellline H22 withretroviralvector LXSN.Results:There were no obvious changesin cell morphology,cellgrowth,cell cycle and DNAcontentof mIFN γgene modified cells.MHC Ⅱantigen expression hadincreasedin H22 mIFN γcellbyindirectfluorometry andthetumorigenicity decreased significantlyin H22 mIFN γcells.Conclusion:Thetransduction of mIFN γgene canincreasetheimmunoge nicity ofhapatoma cells,whichisthe base ofapplication oftumor vaccineto clinicalgenetherapy.