化学发光法定量检测药物光毒性

In vitro Quantitative Assessment of Drug Phototoxicity by a Chemiluminescence Method

目的 建立化学发光法检测药物光毒性的模型。方法 实验中将还原型辅酶I(NADH)与待测药物混合后置于UVA光源 (发射光谱 31 0nm～ 4 0 0nm ,峰值位于 35 0nm处 )下照射 ,一定剂量后加入化学发光剂鲁米诺 ,计数化学发光值 ,以发光值反映光毒反应强度。结果 经不同剂量UVA照射后 ,各种药物和蒸馏水样品发光值均有不同程度的升高 ,发光值与UVA剂量呈直线正相关关系。统计学检验结果显示 :与蒸馏水样品相比 ,吡哌酸、强力霉素和灰黄霉素样品发光值差别有显著性。结论 化学发光法检测到吡哌酸、强力霉素和灰黄霉素的光毒性 ,光毒反应强度在一定范围内随UVA剂量增加而增强。

Objective To establish a test model for phototoxic agents with the method of chemiluminescence.Methods Pipemidic acid,doxycycline,griseofuvin and chlorpromaziine were detected.NADH was irradiated with UVA in the presence of photosensitixing agents,then luminol was added to the test samples,and chemiluminescent value was counted and calculated.Ittadiation of NADH without photosensitizing drugs was used as control.Results Chemiluminescent values of all the samples went up with the increase of UVA dosage.The result showed those chemiluminescent values of pipemidic acid,doxycycline and griseofuvin were significantly higher than that of control.The result of linear regression analysis showed that phototoxic intensity was linear correlated with UVA dosage.Conclusion Phototoxicity of pipemidic acid,doxycycline and griseofuvin can be detected with the method of chemiluminescence.