缺血再灌注的兔肾动脉阻力指数与肾组织TNF-α表达的相关性研究

A correlative study on the resistance index of rabbit renal artery after ischemia-reperfusion and the TNF-α expression in renal tissue

目的探讨肾组织肿瘤坏死因子(TNF-α)表达与肾缺血再灌注(I/R)后肾动脉阻力指数(RI)的相关性,为评价肾缺血再灌注损伤(IRI)的发生提供可行方法。方法将48只大白兔随机分为假手术组、I/R组。应用彩色多普勒血流显像(CDFI)监测各组大白兔主肾动脉(MRA)、段动脉(SRA)、叶间动脉(IRA)的RI变化,免疫组化检测肾组织中TNF-α的表达。结果与假手术组比较,I/R组大白兔再灌注2h肾动脉无明显RI改变,再灌注8h及24h分别出现SRA、IRA的RI增大及MRA、SRA、IRA的RI增大(P<0.05)。I/R组8、24h大白兔肾组织的TNF-α表达水平高于假手术组(P<0.05)。各组MRA、SRA和IRA的RI与肾组织TNF-α表达呈正相关(P<0.05)。结论利用CDFI检测缺血再灌注的肾动脉的RI是一种无创、及时的评价肾IRI发生的可行方法。

Objective To explore the correlation of expression of tumor necrosis factor-alpha（TNF-α） in renal tissue and the resistance index（RI） of renal artery after renal ischemia-reperfusion（I/R）,and provide a feasible method to evaluate the occurrence of renal I/R injury.Methods 48 rabbits were randomly divided into sham operation group and I/R group.Color Doppler flow imaging（CDFI） was adopted to monitor the changes of RI of main renal artery（MRA）,segmental renal artery（SRA） and interlobar renal artery（IRA） of rabbits in each group.Immunohistochemistry was employed to detect the expression of TNF-α in renal tissue.Results Compared with sham operation group,RI of renal artery showed no significant changes in rabbits subjected to 2 hours of reperfusion in I/R group,while that of SRA,IRA and that of MRA,SRA,IRA increased in rabbits subjected to 8 and 24 hours of reperfusion in I/R group,respectively（P0.05）.Level of TNF-α expression in renal tissue of rabbits in I/R 8,24 h group was higher than that in sham operation group（P0.05）.RI of MRA,SRA and IRA in each group had positive correlation with its TNF-α expression in renal tissue（P0.05）.Conclusion Detection the RI of renal artery after I/R by CDFI is a feasible method with non-invasion to evaluate timely the occurrence of renal IRI.