汉桃叶抗炎镇痛作用有效部位的筛选

Screening of anti-inflammatory and analgesic extraction from Schefflera arboricola Hayata

目的分离并筛选汉桃叶抗类风湿性关节炎的有效部位。方法采用系统溶剂提取法对汉桃叶进行提取、分离得到不同部位的提取物。分别采用小鼠二甲苯致耳廓肿胀、大鼠棉球所致肉芽肿、小鼠醋酸扭体法和小鼠热板法评价活性。结果乙酸乙酯提取物对小鼠耳廓肿胀抑制率为50.10%,有显著抗炎作用(P<0.01);对大鼠棉球所致肉芽肿的抑制率为56.05%,有显著抗炎作用(P<0.01);对醋酸致小鼠疼痛的扭体抑制率达50.17%,有明显镇痛作用(P<0.01);对热板所致的小鼠疼痛抑制率为55.01%,有显著镇痛作用(P<0.01)。结论汉桃叶乙酸乙酯提取物可能是其治疗类风湿性关节炎的有效部位。

Aim To separate and screen the effective parts of anti-rheumatoid of Schefflera arboricola Hayata.Methods The different parts of the Schefflera arboricola Hayata were extracted and separated by the methods of system solvent extraction.Four models were used to explore the anti-inflammatory and analgesic effect of Schefflera arboricola Hayata.They were ear swelling caused by Dimethyl benzene in mice,granulation tissue was established by cotton pellets implantation in rats,hot plates and writhing test in mice.Results For Ethyl acetate extract,the inhibition rate of ear swelling caused by Dimethyl benzene in mice was 50.10%,showing a good anti-inflammatory effect（P〈0.01）.The inhibition rate of ranulation tissue established by cotton pellets implantation in rats was 56.05%,shown a good anti-inflammatory effect（P〈0.01）.The inhibition rate of writhing test in mice was 50.17%,showing a striking analgesic effect（P〈0.05）.The inhibition rate of hot plate in mice was 55.01%,showing a good analgesic effect（P〈0.01）.Conclusion Ethyl acetate extract may be the effective parts of Schefflera arboricola Hayata for rheumatoid arthritis.