HPLC测定益血糖浆中人参皂苷R_(b1)、R_(g1)、Re的含量

Determination of content of ginsenoside-Rb_1、Rg_1、Re in Yixue syrup by Rp-HPLC

目的测定益血糖浆中人参皂苷Rb1、Rg1、Re的含量。方法高效液相色谱法。色谱柱Agilent TC-C18（4.6mm×250mm,5μm）,流动相采用梯度洗脱,A为乙睛,B为0.05%磷酸水溶液,0～50min（20：80）;50～60min（20→29：80→71）;60～110min（29：71）,检测波长203nm,体积流量1.0mL/min,柱温40℃。结果人参皂苷Rb1在1.0~5.0μg范围内呈良好线性（r=0.9999,n=5）,平均回收率为（99.08%）;人参皂苷Rg1在0.7~3.5μg范围内呈良好线性（r=0.9999,n=5）,平均回收率为（99.63%）;人参皂苷Re在0.6~3.0μg范围内呈良好线性（r=0.9999,n=5）,平均回收率为（100.7%）。结论本方法简便、准确、可靠,可用于益血糖浆中人参皂苷Rb1、Rg1、Re的含量测定。

Objective To develop a method for determination of content of ginsenoside Rg1、Re in Yixue syrup by HPLC.Method The Hypersil ODS column（5mm,4.6mm×250mm） was used,eluted with acetonitrile-0.2% phosphoric acid water in gradient mode.he UV detection wavelength was 203 nm.and the flow rate was 1.0mL/min,with the tempture of column at 40℃.Results The good linear relationship in the range of 1.0 to 5.0μg for Ginsenoside Rb1（r=0.9999,n=5）,and the recovery ratio is（99.08%）,Also The good linear relationship in the range of 0.7 to 3.5μg for Ginsenoside Rg1（r=0.9999,n=5）,and the recovery ratio is（99.63%）,Also the good linear relationship for Ginsenoside Re in the range of 0.6 to 3.0μg（r=0.9999,n=5） was obtained.The recovery ratio was（100.7%）.Conclusion The method is simple,accurate and reliable.Fit to determination of content of ginsenoside-Rb1、Rg1、Re in Yixue syrup by HPLC.