HPCE法和HPLC法测定蒲黄中黄酮甙的含量

Determination of Flavonoids in Pollen Typhae(Puhuang) by HPCE and HPLC

目的：采用高效毛细管电泳法和高效液相色谱法测定蒲黄中异鼠李素３Ｏ新橙皮糖甙和香蒲新甙的含量。方法：ＨＰＣＥ 用缓冲液０－０２ｍｏｌ·Ｌ－ １ 硼砂０－０５ｍｏｌ·Ｌ－ １ 十二烷基硫酸钠的１０ ％ 乙腈，电压２０ｋＶ，柱温３０ ℃，检测波长２７０ｎｍ ，进样量３０ｋＰａ·ｓ；ＨＰＬＣ 用Ｃ１８ 柱，流动相乙腈水（１５∶８５） ，检测波长２５４ｎｍ 。结果：两种黄酮甙的含量在０－１ ％ ～０－６ ％ 。结论：两种方法均可用于蒲黄的质量控制。

Objective:To determine two flavonoids isorhamnetin 3 O neohesperidoside(Ⅰ) and typheneoside(Ⅱ) in Pollen Typhae by HPCE and HPLC.Method:HPCE conditions:buffer=0.02mol·L -1 borate,0.05mol·L -1 SDS and 10% acetonitrile;V=20kV;T=30℃;λ=270nm;sample size=30kPa·s,HPLC conditions:C 18 lumn,acetonitrile water(15∶85) as mobile phase ditection wavelength at 254nm.Result:The contents of flavonoids I and II in five different Typha species and eighteen commercial samples of traditional Chinese drug Puhuang were between 0.1%～0.6%.Conclusion:These two methods are rapid,simple,accurate and can be used for the quality control of this traditional Chinese drug.