内源性乳糖结合凝集素在胃癌中的表达

PRELIMINARY RESEARCH ON EXPRESSION OF ENDOGENOUS LACTOSE BINDING LECTIN IN GASTRIC CARCINOMA

目的：探讨内源性乳糖结合凝集素在胃癌中的表达及其意义。方法：采用亲和层析法分离纯化胃癌内源性乳糖结合凝集素，免疫家兔制备多克隆抗体，免疫组织化学方法（ＡＢＣ法）检测胃癌组织内源性乳糖结合凝集素表达。结果：纯化得到一分子量为３０ ｋＤ的内源性乳糖结合凝集素，免疫组化检测发现胃癌乳糖结合凝集素表达阳性率为３２．５％ ，Ⅰ、Ⅱ、Ⅲ期阳性率分别为２０．０％ 、２９．６％ 和５０．０％ （Ｐ＞ ０．０５），组织学高分化与低分化者阳性率分别为２５．０％ 和４３．８％ （Ｐ＞ ０．０５）。结论：内源性乳糖结合凝集素可能与胃癌的进展。

Objective:To investigate the expression of endogenous lactose binding lectin in human gastric carcinoma.Methods:Endogenous lactose binding lectin was purified by affinity chromatography on lactose sepharose 4B column.Rabbits were immunized with purified lectin,polyclonal antibody against endogenous lactose binding lectin were purified by protein A affinity chromatography.Expression of endogenous lactose binding lectin in tissues were detected immunohistochemically by avidin biotin peroxidase complex.Results:The positive staining rate of endogenous lactose binding lectin in gastric carcinoma was 32.5%.In Ⅰ,Ⅱ and Ⅲ stage diseases,the positive rates were 20.0%,29.6% and 50.0% respectively ( P >0 05).The positive rate (43 8%) in histologically poorly differentiated group was higher than that (25%) in well differentiated group( P >0 05).Conclusion:Endogenous lactose binding lectin may be involved in the progress,metastasis and recurrence of gastric carcinoma.