54株分枝杆菌国际标准株16S-23S rRNA转录间隔区序列分析

Analysis of the genotype of 54 reference strains of Mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences

目的分析54株分枝杆菌国际标准株16S-23SrRNA转录间隔区(ITS)序列,为临床分离株的鉴定提供参考。方法用16S-23SrRNA ITS序列分析法对54株分枝杆菌国际标准株进行分析,构建系统发育树,计算相似性百分比。结果除灰尘与微黄分枝杆菌;田野与千田分枝杆菌;抗热与副偶然分枝杆菌,奥布与母牛结核分枝杆菌;杜氏与猪分枝杆菌;金色与东海分枝杆菌,海与溃疡分枝杆菌、科莫斯分枝杆菌;2株结核分枝杆菌与田鼠分枝杆菌、非洲分枝杆菌的16S-23SrRNA ITS序列完全相同无法鉴别外,其他各分枝杆菌菌种间16S-23SrRNAITS序列均不相同,可以得到很好的鉴别。结论 16S-23SrRNA ITS序列分析是一种很好的鉴定分枝杆菌的方法,国际标准株16S-23SrRNA ITS序列的研究弥补了基因数据库的不足。

Objective To analyze genotype of 54 reference strains of mycobacteria by 16S-23S rRNA Internal Transcription Space（ITS） sequence analysis.Methods The genotype of 54 reference strains of mycobacteria were analyzed by 16S-23S rRNA ITS sequence analysis.Then the phylogenetic tree was constructed and calculated similarly.Results The sequencing of 16S-23S rRNA ITS of the 54 reference strains of mycobacteria was finished.All the mycobacteria could be discriminated except that M.flavescens and M.pulveris,M.agri and M.chitae,M.thermoresistibile and M.parafortuitum,M.obuense and M.vaccae,M.duvalii and M.porcinum,M.aurum and M.sphagni,M.ulcerans and M.marinum,M.komossense,2 strains of M.tuberculosis and M.microti,M.africanum.Conclusion 16S-23S rRNA ITS sequence analysis is a reliable method to discriminate mycobacteria.The study of the genotype of the 54 reference strains of Mycobacteria is a compensation for the gene database.