骨髓增生异常综合征患者血清细胞因子表达及相关性研究

Study on the expression and correlation of serum cytokines in patients with myelodysplastic syndrome

目的探讨血清肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)和IL-12水平与骨髓增生异常综合征(MDS)的关系及临床意义,进而研究其在MDS发病中的作用,为临床防治MDS提供理论依据。方法采用酶联免疫吸附试验(ELISA)检测40例MDS患者及40例健康人血清中TNF-α、IL-10和IL-12水平。结果与健康对照组比较,MDS患者血清TNF-α、IL-10和IL-12水平明显升高,差异有统计学意义(P<0.01);观察MDS不同分型间血清TNF-α、IL-10和IL-12水平发现,MDS早期组和进展组患者血清TNF-α、IL-10和IL-12水平均高于健康对照组,差异有统计学意义(P<0.01),且进展组血清IL-10水平显著高于早期组,差异有统计学意义(P<0.01),但进展组血清TNF-α和IL-12水平低于早期组,差异有统计学意义(P<0.01)。结论 TNF-α、IL-10和IL-12参与MDS发病及病情演变,检测TNF-α、IL-10和IL-12有助于指导MDS分型和判断预后,并为治疗MDS提供新思路。

Objective To explore the relation between serum TNF-α,IL-10,IL-12 levels and myelodysplastic syndrome and its clinical significance.Then to study its role in the pathogenesis of MDS,which providing a theoretical basis for the prevention and treatment of MDS.Methods Serum levels of TNF-α,IL-10 and IL-12 were measured with ELISA in 40 cases of patients with myelodysplastic syndrome and 40 healthy subjects taken as control group.Results Serum TNF-α,IL-10,IL-12 levels of myelodysplastic syndrome were significantly higher than those of the healthy control group（all P0.01）.We observed the serum TNF-α,IL-10 and IL-12 levels in different types of MDS.The results showed that the serum TNF-α,IL-10 and IL-12 levels in prophase group and progression group were significantly increased compared with the level of the control group（P0.01）.IL-10 level in progression group of MDS was higher than that in the prophase group（P0.01）;TNF-α and IL-12 levels in the progression group of MDS were lower than those in the prophase group（P0.01）.Conclusion TNF-α,IL-10 and IL-12 take part in the pathogenesis of MDS.Detection of TNF-α,IL-10 and IL-12 may be helpful for typing and predicting its prognosis of MDS.In addition,it may provide new clues for the treatment of MDS.