M2型肿瘤相关巨噬细胞在上皮性卵巢癌中的检测

Detection of M2-polarized tumor-associated macrophages in human epithelial ovarian cancer

目的:探讨M2型肿瘤相关巨噬细胞(TAMs)在上皮性卵巢癌组织中的分布,及其与临床病理特征及淋巴管(LV)生成的关系。方法:本研究共纳入74例样本,41例卵巢癌为实验组,18例交界性及15例良性肿瘤为对照组,采用免疫组化方法,用CD68标记巨噬细胞(CD68+)、CD163标记M2型TAMs(CD163+)、D2-40标记LV,了解其在实验组和对照组的分布,及与卵巢癌临床病理特征的关系。结果:卵巢癌组CD68+细胞数、CD163+细胞数、LV数均多于交界性和良性肿瘤组(P<0.01)。CD68+细胞分布于癌巢和癌间质,CD163+细胞和LV主要分布于癌间质,癌间质中三者数量明显多于癌巢(P<0.01)。癌巢和癌间质中CD68+、CD163+细胞数及癌间质LV数与临床分期、组织学分化、病理类型、手术切缘累及淋巴结转移有关(P<0.01)。癌间质LV数与CD68+和CD163+细胞数相关(r=0.759,P<0.01;r=0.888,P<0.01)。结论:卵巢癌组织中TAMs主要为M2型,主要分布于癌间质。其细胞数与肿瘤的临床分期、组织分化、病理类型、手术切缘累及和淋巴结转移有关,提示TAMs可能通过促进淋巴管生成,利于肿瘤的淋巴转移。

Objective：To investigate the distribution of M2-Polarized tumor-associated macrophages（TAMs）in human epithelial ovarian cancer as well as its correlation with clinical pathological features and lymphangiogenesis.Methods：Seventy-four samples were enrolled in this study,including 41 samples of patients diagnosed with ovarian cancer as the experimental group,18 samples with borderline ovarian tumors and 15 samples with benign ovarian tumors as the control group.We used CD68 to mark macrophages and CD163 to mark M2-polarized TAMs as well as D2-40 to mark lymphatic vessels（LV）in immunohistochemistry study,to explore the correlation between macrophages distribution and clinical pathological features in these samples.Result：CD68 positive macrophages were detected in both tumor islets and stroma,while CD163-positive TAMs and LV were mainly distributed in the stroma with relatively lower number detected in tumor islets（P0.01）.Numbers for CD68＋,CD163＋ and D2-40＋ cels in ovarian cancer were significantly higher than those in the benign and boradline group（P0.01）.The number of CD68＋ macrophages,M2-polarized TAMs and LV in tumor islets and stroma were closely related to the clinical stage,histological differentiation,pathological subtypes,the surgical magrin involved and lymph node metastasis.They were all statistically significant different（P0.01）.The lymph-vessel number in the tumor stroma was related with that of CD68＋ macrophages and CD163＋ macrophages（r=0.759,P0.01;r=0.888,P0.01）.Conclusion：TAMs in overian cancer are predominantly M2 type,mainly distributed in tumor stroma,which are related with clinical stage,histological differentiation,pathological subtypes,the surgical magrin involved and lymph node metastasis of the tumor.The data indicate that TAMs may advocate LV formation which may assist tumor metastasis.