膜联蛋白Ⅳ在卵巢透明细胞癌和浆液性囊腺癌中的差异表达

The different expression of annexin Ⅳ between ovarian clear cell carcinoma and ovarian serous cystadenocarcinoma

目的:通过建立基因表达谱,研究透明细胞癌和浆液性囊腺癌之间差异表达基因。方法:利用cDNA芯片建立和分析两者基因表达谱,并对透明细胞癌中上调表达的膜联蛋白Ⅳ,在组织和细胞中进行了定量PCR以及免疫组织化学和Western blot验证。结果:卵巢透明细胞癌和卵巢浆液性囊腺癌基因表达谱比较,差异2倍以上基因共有82个,25个表达上调,57个表达下调。其中有功能的上调基因7个,下调基因12个。组织和细胞中膜联蛋白Ⅳ在mRNA及蛋白水平上的表达与基因芯片结果一致。结论:通过基因芯片可以建立卵巢透明细胞癌和浆液性囊腺癌的差异表达基因谱,膜联蛋白Ⅳ在卵巢透明细胞癌中上调表达可能具有重要意义。

Objective：To study the genes that differently expressed between the ovarian clear cell carcinoma and the serous cystadenocarcinoma by the gene expression profile.Method：Using the cDNA microarray,the gene expression profile between clear cell carcinoma and serous cystadenocarcinoma was analyzed.One of the up-regulated genes,annexin Ⅳ was chosen to be verified by real time PCR and immunohistochemistry as well as Western blot analysis in cells and tissue.Results：Eighty-two genes had more than two-fold different expression.The function of 7 up-regulated genes and 12 down-regulated genes were identified.The expression of annexin Ⅳ in mRNA and protein level was in accordance with the result of microarray.Conclusion：The gene expression profile contributes to the exploration of the genes that are differential expressed between the two types of ovarian carcinoma,and elevated expression of annexin Ⅳ in ovarian clear cell carcinoma may be of great significance.